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KMID : 0363720080410010073
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Korean Journal of Anatomy
2008 Volume.41 No. 1 p.73 ~ p.79
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Different Expressions of eNOS and AQP4 after Focal Cerebral Ischemia in Rat: In Vasogenic Edema
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Park Eun-Jee
Jung Yong-Wook
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Abstract
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Brain edema, the infiltration and accumulation of excess fluid causing an increase in brain tissue volume, often leads to a rise in intracranial pressure (ICP) and is a key contributor to the morbidity and mortality associated with brain injury. We examined the mechanisms for vasogenic brain edema produced by focal cerebral ischemia. To test the mechanisms underlying vasogenic brain edema, we compared the expression of endothelial nitric oxide synthase (eNOS) and aquaporin-4 (AQP4) in the penumbra of brain tissues after focal cerebral ischemia. The expression of eNOS and AQP4 in the ischemic penumbra was determined by quantitative immunoblot analysis. In focal cerebral ischemia, eNOS xpression increased significantly to 129%±6% of the value for sham-operated controls (n=4, P?0.01). In contrast, AQP4 expression decreased significantly to 52%±3% of the value for shamoperated controls (n=4, P?0.01). These findings suggest that the expression of eNOS and AQP4 in the focal cerebral ischemia may play different roles in the formation of vasogenic brain edema. Additionally, a larger decrease in the neuronal nuclei (NeuN) (39%±7% of the value in sham-operated controls; n=4, P?0.01) than the decrease in glial fibrillary acidic protein (GFAP) (53%±14% of sham-operated controls; n=4, P?0.01) in the penumbra after focal cerebral ischemia suggests that the neurons are more susceptible to ischemic injury than are the astrocytes.
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KEYWORD
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Brain edema, Focal cerebral ischemia, eNOS, AQP4
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